Silver: 50x TAE: Difference between revisions
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50x TAE is 2M Tris, 2M Acetic Acid | The final concentrations of reagents in 50x TAE is 2M Tris, 2M Acetic Acid, and 50mM EDTA. | ||
Per 1L: | Per 1L: | ||
*242g Tris base | *242g Tris base | ||
*57.1 ml acetic acid | *57.1 ml acetic acid (glacial) | ||
*100ml 0.5 M EDTA | *100ml 0.5 M EDTA | ||
'''DO NOT AUTOCLAVE''' | '''DO NOT AUTOCLAVE''' | ||
[[Category:Material]] | |||
[[Category:Buffers]] | |||
[[Category:Agarose gel electrophoresis]] | |||
Latest revision as of 17:42, 20 March 2013
The final concentrations of reagents in 50x TAE is 2M Tris, 2M Acetic Acid, and 50mM EDTA.
Per 1L:
- 242g Tris base
- 57.1 ml acetic acid (glacial)
- 100ml 0.5 M EDTA
DO NOT AUTOCLAVE
