Silver: 50x TAE

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(Arrangement of words in the the final concentrations sentence could lead to confusion (2M Acetic Acid called "glacial"))
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50x TAE is 2M Tris, 2M Acetic Acid (glacial), and 50mM EDTA in its final 1L volume.
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The final concentrations of reagents in 50x TAE is 2M Tris, 2M Acetic Acid, and 50mM EDTA.
Per 1L:
Per 1L:
*242g Tris base
*242g Tris base
-
*57.1 ml acetic acid
+
*57.1 ml acetic acid (glacial)
*100ml 0.5 M EDTA
*100ml 0.5 M EDTA
'''DO NOT AUTOCLAVE'''
'''DO NOT AUTOCLAVE'''

Revision as of 11:08, 19 December 2006

The final concentrations of reagents in 50x TAE is 2M Tris, 2M Acetic Acid, and 50mM EDTA.

Per 1L:

  • 242g Tris base
  • 57.1 ml acetic acid (glacial)
  • 100ml 0.5 M EDTA

DO NOT AUTOCLAVE

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