Silver: 50x TAE
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(Difference between revisions)
(Arrangement of words in the the final concentrations sentence could lead to confusion (2M Acetic Acid called "glacial")) |
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| - | 50x TAE is 2M Tris, 2M Acetic Acid | + | The final concentrations of reagents in 50x TAE is 2M Tris, 2M Acetic Acid, and 50mM EDTA. |
Per 1L: | Per 1L: | ||
*242g Tris base | *242g Tris base | ||
| - | *57.1 ml acetic acid | + | *57.1 ml acetic acid (glacial) |
*100ml 0.5 M EDTA | *100ml 0.5 M EDTA | ||
'''DO NOT AUTOCLAVE''' | '''DO NOT AUTOCLAVE''' | ||
Revision as of 11:08, 19 December 2006
The final concentrations of reagents in 50x TAE is 2M Tris, 2M Acetic Acid, and 50mM EDTA.
Per 1L:
- 242g Tris base
- 57.1 ml acetic acid (glacial)
- 100ml 0.5 M EDTA
DO NOT AUTOCLAVE
