Silver: Commonly Used Primers: Difference between revisions
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== Commonly Used Primers == | == Commonly Used Primers == | ||
* Sequencing primers for Biobrick vectors | * Sequencing primers for Biobrick & Biofusion vectors: | ||
** pSB1A3, pSB1AC3, pSB1AK3, pSB1AT3 forward primer: 5' AAATAGGCGTATCACGAGGC 3' | |||
** pSB1A3, pSB1AC3, pSB1AK3, pSB1AT3 reverse primer: 5' GAGTCAGTGAGCGAGGAAGC 3' | |||
** V0100/V0120 forward sequencing primer: 5' GGGTTTTCCCAGTCACGACG 3' | |||
** V0100/V0120 reverse sequencing primer: 5' TGTGGAATTGTGAGCGGATAACA 3' | |||
** V0002 forward sequencing primer: 5' tttctggaattcgcggcc 3' | ** V0002 forward sequencing primer: 5' tttctggaattcgcggcc 3' | ||
** V0002 reverse sequencing primer: 5' gccggactgcagcggcc 3' | ** V0002 reverse sequencing primer: 5' gccggactgcagcggcc 3' | ||
* Primers that sequence regions between the Biobrick ends | * Primers that sequence regions between the Biobrick ends | ||
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** upstream of midpoint btwn yeast-codon optimized YFP/mCherryx2(Z0035 or Z0037): 5' GCTAGTTGCTCTAGTTGCGGTACC 3' | ** upstream of midpoint btwn yeast-codon optimized YFP/mCherryx2(Z0035 or Z0037): 5' GCTAGTTGCTCTAGTTGCGGTACC 3' | ||
* Sequencing primers for | * Sequencing primers for other vectors | ||
pET vectors 5' atgcgtccggcgtaga | |||
T7 promoter 5' taatacgactcactataggg | |||
T7 terminator 5' gctagttattgctcagcgg | |||
*'''[[User:CarolineAjo-Franklin|CAjoF]] 20:27, 29 January 2008 (CST)''': | |||
Revision as of 19:27, 29 January 2008
Commonly Used Primers
- Sequencing primers for Biobrick & Biofusion vectors:
- pSB1A3, pSB1AC3, pSB1AK3, pSB1AT3 forward primer: 5' AAATAGGCGTATCACGAGGC 3'
- pSB1A3, pSB1AC3, pSB1AK3, pSB1AT3 reverse primer: 5' GAGTCAGTGAGCGAGGAAGC 3'
- V0100/V0120 forward sequencing primer: 5' GGGTTTTCCCAGTCACGACG 3'
- V0100/V0120 reverse sequencing primer: 5' TGTGGAATTGTGAGCGGATAACA 3'
- V0002 forward sequencing primer: 5' tttctggaattcgcggcc 3'
- V0002 reverse sequencing primer: 5' gccggactgcagcggcc 3'
- Primers that sequence regions between the Biobrick ends
- upstream of a Kozak region (K0001): 5' TCTAGTCTCCATGGTGGCGG 3'
- downstream of a Kozak region (K0001): 5' CCGCCACCATGGAGACTAGA 3'
- upstream of the ADH1 terminator (L0100): 5' CCTGAGAAAGCAACCTGACCTACAG 3'
- downstream of midpoint btwn yeast-codon optimized YFP/mCherryx2 (Z0035 or Z0037): 5' GGTACCGCAACTAGAGCAACTAGC 3'
- upstream of midpoint btwn yeast-codon optimized YFP/mCherryx2(Z0035 or Z0037): 5' GCTAGTTGCTCTAGTTGCGGTACC 3'
- Sequencing primers for other vectors
pET vectors 5' atgcgtccggcgtaga T7 promoter 5' taatacgactcactataggg T7 terminator 5' gctagttattgctcagcgg
- CAjoF 20:27, 29 January 2008 (CST):
