Silver: Dropout Plates

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Revision as of 11:32, 5 July 2006 by JulianEskin (Talk | contribs)
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Dropout Plates (per 1L)

  • In 500 mL bottle:
    • 6.7g yeast nitro base
    • 2.0g dropout powder
    • 200 mL H2O
  • In 2L flask:
    • 20g agar
    • 700mL H2O
  • Autoclave both, and also autoclave a stir bar in a separate beaker
  • Add 100 mL 20% glucose to the 500 mL bottle.
  • Add the autoclaved stir bar to the 2L flask.
  • Let cool, add contents of bottle to flask.
  • Stir until cool to the touch.
  • Pour plates.
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