Silver: Protocols: Difference between revisions
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*[[Silver: YEPD Liquid|YEPD Liquid]] | *[[Silver: YEPD Liquid|YEPD Liquid]] | ||
*[[Silver: YEPD Plates|YEPD Plates]] | *[[Silver: YEPD Plates|YEPD Plates]] | ||
==Hardware_Maintenance== | |||
*[[Silver: Microscope|Microscope]] | |||
Revision as of 16:33, 26 February 2010
See also the OpenWetWare Protocols Collection.
Silver Lab Members: Link to your protocols from both this page and the OpenWetWare Protocols page.
Back to Silver Lab
Genome Organization
- S. cerevisiae Protocols
- Mammalian Protocols
RNA
Synthetic Biology
- Strategy for Making Parts
- Commonly Used Plasmids
- Commonly Used Primers
- PCR
- Oligonucleotide Inserts
- Overlapping Oligonucleotide Inserts
- Site-Directed Mutagenesis
- Restriction Digest
- Ligation
- Quick & Dirty Digest & Ligation
- Bacterial Transformation
- Plasmid Verification
- Integration into Yeast
- Verifying Genomic Integration
Cell-Based Screens
Methylation
Other / More General
S. cerevisiae Protocols
Mammamlian Tissue Culture Protocols
Other Protocols
Common Reagents
- Ampicillin
- B Plates
- Coomassie Stain
- Destain
- Dropout Media
- Dropout Plates
- 0.5 M EDTA pH 8.0
- 20% Glucose
- 50% Glycerol
- LB Liquid
- LB-Amp Plates
- 10x PBS
- Protease Inhibitors
- ssDNA
- SOC
- 50x TAE
- 1M Tris Base
- TE
- YEPD Liquid
- YEPD Plates
