Silver: Protocols

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Genome Organization

• S. cerevisiae Protocols
  • ChIP-chip
  • FISH/IF
  • Immunofluorescence
  • LiOAc Transformation
  • Propidium Iodide FACS
• Mammalian Protocols

RNA "Stuff"

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Synthetic Biology

• BioBrick Strategy
• PCR
• Oligonucleotide Inserts
• Restriction Digest
• Bacterial Colony PCR
• Ligation
• Bacterial Transformation
• Plasmid Verification

Cell-Based Screens

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Methylation

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Other / More General

S. cerevisiae Protocols

• Lysate for Western
• Colony PCR
• Time-Lapse Microscopy

Other Protocols

Common Reagents

• Silver: Ampicillin
• Silver: B plates
• Silver: Coomassie Stain
• Silver: Destain
• Silver: Dropout Media
• Silver: Dropout Plates
• Silver: Dropout Powders
• 0.5 M EDTA pH 8.0
• Silver: FOA Plates
• Silver: G418 plates
• Silver: 20% Glucose
• Silver: 50% Glycerol
• Silver: LB Liquid
• Silver: LB Plates
• Silver: 10x PBS
• Silver: RNase 10mg/ml
• Silver: SOC
• Silver: ssDNA
• Silver: 50x TAE
• Silver: 1M Tris Base
• Silver: TE
• Silver: YEPD Liquid
• Silver: YEPD Plates