Silver: RNA Dynamics

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===Dynamics of RNAs===
===Dynamics of RNAs===
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The importance of RNAs in regulation of cell determination and disease continues to grow. We study the dynamics of RNAs including co-transcriptional alternative splicing and transport, and the relative efficiency of pre-mRNA processing and its impact on noise in gene expression. ([[User: IanSwinburne |Ian Swinburne]]). We use a combination of genetic, biochemical and novel genomic and imaging approaches to study RNA dynamics on a systems-wide level in both model organisms (yeast and fly) and human cells, eg ([[User: NatalieGilks |Natalie Gilks]], [[User: OonaJohnstone |Oona Johnstone]], and [[User: MichaelMoore |Michael Moore]]). We have generated a spatial and temporal map of the expression of all RNA-binding proteins in mammalian neural development ([[User: AdrienneMckee |Adrienne Mckee]]).  One goal is to decode the way that proteins recognize RNA throughout the genome.
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The importance of RNAs in regulation of cell determination and disease continues to grow. We study the dynamics of RNAs including co-transcriptional alternative splicing and transport out of the nucleus, as well as proper localizatin in the cytoplasm. We use both yeast and mammalian cells.  In doing so, we have discovered that mRNAs are processed and recruited for transport as they are synthesized.  This finding has allowed us to employ genome-wide location analysis to determine the patterns of alternative splicing and the specificity of proteins that affect RNA processing and movement. We have also generated a spatial and temporal map of the expression of all RNA-binding proteins in mammalian neural development.  The mouse RNA binding protein data is posted [http://www.informatics.jax.org/searches/reference.cgi?104515 here].
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The mouse RNA binding protein data is posted [http://www.informatics.jax.org/searches/reference.cgi?104515 here].
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Recent studies include the identification of all metazoan proteins important for mRNA transport and for alternative splicing of genes important in certain diseases and physiological changes such as apoptosis. In addition, in combination with our synthetic biology efforts, we are building novel genes to examine the impact of gene and intron length on gene expression. Certain models predict interesting behavior such as oscillations depending on the gene structure that could  impact how genes are regulated during development.

Revision as of 07:31, 28 March 2007

Dynamics of RNAs

The importance of RNAs in regulation of cell determination and disease continues to grow. We study the dynamics of RNAs including co-transcriptional alternative splicing and transport out of the nucleus, as well as proper localizatin in the cytoplasm. We use both yeast and mammalian cells. In doing so, we have discovered that mRNAs are processed and recruited for transport as they are synthesized. This finding has allowed us to employ genome-wide location analysis to determine the patterns of alternative splicing and the specificity of proteins that affect RNA processing and movement. We have also generated a spatial and temporal map of the expression of all RNA-binding proteins in mammalian neural development. The mouse RNA binding protein data is posted here.

Recent studies include the identification of all metazoan proteins important for mRNA transport and for alternative splicing of genes important in certain diseases and physiological changes such as apoptosis. In addition, in combination with our synthetic biology efforts, we are building novel genes to examine the impact of gene and intron length on gene expression. Certain models predict interesting behavior such as oscillations depending on the gene structure that could impact how genes are regulated during development.

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