Silver: RNA Dynamics

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(Dynamics of RNAs)
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===Dynamics of RNAs===
===Dynamics of RNAs===
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The importance of RNAs in regulation of cell determination and disease continues to grow. We study the dynamics of RNAs including co-transcriptional alternative splicing and transport out of the nucleus, as well as proper localizatin in the cytoplasm.  We use both yeast and mammalian cells.  In doing so, we have discovered that mRNAs are processed and recruited for transport as they are synthesized.  This finding has allowed us to employ genome-wide location analysis to determine the patterns of alternative splicing and the specificity of proteins that affect RNA processing and movement.  We have also generated a spatial and temporal map of the expression of all RNA-binding proteins in mammalian neural developmentThe mouse RNA binding protein data is posted [http://www.informatics.jax.org/searches/reference.cgi?104515 here].
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The importance of RNAs in regulation of cell determination and disease continues to grow. We study the dynamics of RNAs including alternative splicing and transport out of the nucleus, as well as proper localization in the cytoplasm.  Much of our past work has employed the simple yeast.  We have more recently extended lessons from yeast to mammalian cells.   
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Recent studies include the identification of all metazoan proteins important for mRNA transport and for alternative splicing of genes important in certain diseases and physiological changes such as apoptosis.  In addition, in combination with our synthetic biology efforts, we are building novel genes to examine the impact of gene and intron length on gene expression.  Certain models predict interesting behavior such as oscillations depending on the gene structure that could  impact how genes are regulated during development.
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We have employed genome-wide location analysis to determine the patterns of alternative splicing and the specificity of proteins that affect RNA processing and movement.  We have also generated a spatial and temporal map of the expression of all RNA-binding proteins in mammalian neural development.  The mouse RNA binding protein data is posted [http://www.informatics.jax.org/searches/reference.cgi?104515 here].
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Recent studies include the identification of all metazoan proteins important for mRNA transport and for alternative splicing of genes important in certain diseases and physiological changes such as apoptosis.  From our siRNA screens, we have identified factors that connect different levels of RNA processing and translation.  In addition, in combination with our synthetic biology efforts, we have built novel gene circuits to examine the impact of intron length on gene expression.  Certain models predict interesting behavior such as oscillations depending on the gene structure that could  impact how genes are regulated during development.

Revision as of 08:32, 14 February 2009

Dynamics of RNAs

The importance of RNAs in regulation of cell determination and disease continues to grow. We study the dynamics of RNAs including alternative splicing and transport out of the nucleus, as well as proper localization in the cytoplasm. Much of our past work has employed the simple yeast. We have more recently extended lessons from yeast to mammalian cells.

We have employed genome-wide location analysis to determine the patterns of alternative splicing and the specificity of proteins that affect RNA processing and movement. We have also generated a spatial and temporal map of the expression of all RNA-binding proteins in mammalian neural development. The mouse RNA binding protein data is posted here.

Recent studies include the identification of all metazoan proteins important for mRNA transport and for alternative splicing of genes important in certain diseases and physiological changes such as apoptosis. From our siRNA screens, we have identified factors that connect different levels of RNA processing and translation. In addition, in combination with our synthetic biology efforts, we have built novel gene circuits to examine the impact of intron length on gene expression. Certain models predict interesting behavior such as oscillations depending on the gene structure that could impact how genes are regulated during development.

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