- ssDNA is stored in cold box (dessicated)
- weigh out enough for 10 mg/ml in dH2O
- stir at room temperature until dissolved (takes a long time)
- shear DNA by passing 6 times through an 18-gauge needle and 6 times through a 22-gauge needle
- bring up to 3x volume with dH20, and then boil solution until it is back at 1x volume.
- aliquot into 1.5 ml Eppendorf tubes and store at –20 °C.