Size selective DNA precipitation

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 15: Line 15:
*Centrifuge which can to upto 10.000 rcf (=g)
*Centrifuge which can to up to 10.000 rcf (=g)
*Appropriate tubes for the centrifuge
*Appropriate tubes for the centrifuge

Revision as of 18:33, 27 August 2008



Kersten S. Rabe

Anyone should feel free to add themselves as a curator for this consensus protocol. You do not need to be a curator in order to contribute. The OpenWetWare community is currently discussing the idea of protocol curators. Please contribute.


A very fast and easy method for the size-selective removal of smaller DNA from larger fragments. By adjusting the PEG and MgCl2 concentration the range of precipitated DNA fragments can be adjusted.



  • DNA to be separated
  • 30% (w/v) PEG 8000/30 mM MgCl2 (concentration of PEG 8000 can be varied to shift the size of the percipitated DNA)
  • TE Buffer, pH 8.0 (10 mM TRIS-HCl, 1 mM EDTA, pH 8.0)


  • Centrifuge which can to up to 10.000 rcf (=g)
  • Appropriate tubes for the centrifuge
  • Pipettes


Critical steps




Acnkowledge any help you had in development, testing, writing this protocol.


See OpenWetWare:Biblio for information on how to reference within a wiki.

Specific Protocols

Add links to all the OWW protocols that have been used in making the consensus.


You can discuss this protocol.

Tag this page with categories to allow easier indexing and searching. See Categories for information on existing categories.

Personal tools