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| [[Skatebro:Project08]]
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| ==Tips==
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| *Don't forget to label
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| *Doublecheck that antibiotics are in stock
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| ==Steps==
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| #Set water bath to 50 degrees
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| #Obtain 500 ml 1.2% LB agar from the media room
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| #Melt LB in microwave:
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| #*loosen the cap a lot, lay down paper towels in case of spill, use 50% power (enter time, press Power, 5, Start)
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| #*monitor as you melt, takes approx. 10 minutes per bottle (if doing 2 or 3 bottles simultaneously, add 5 minutes)
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| #Cool in waterbath to 50-60 degrees
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| #Add antibiotics and swirl: (for 500mL of LB Agar)
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| #*500μL Amp stock (Amp stock = 50mg/ml)
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| #*1mL Kan stock (Kan stock = 10mg/ml)
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| #*750μL Gent stock
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| #*500μL Tet stock
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| #*500μL Chl stock
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| #Pour, let stand, label, store in fridge
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