Skatebro:Pouring Plates

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Current revision (06:07, 9 February 2008) (view source)
(Removing all content from page)
 
Line 1: Line 1:
-
[[Skatebro:Project08]]
 
-
==Tips==
 
-
*Don't forget to label
 
-
*Doublecheck that antibiotics are in stock
 
-
 
-
==Steps==
 
-
#Set water bath to 50 degrees
 
-
#Obtain 500 ml 1.2% LB agar from the media room
 
-
#Melt LB in microwave:
 
-
#*loosen the cap a lot, lay down paper towels in case of spill, use 50% power (enter time, press Power, 5, Start)
 
-
#*monitor as you melt, takes approx. 10 minutes per bottle (if doing 2 or 3 bottles simultaneously, add 5 minutes)
 
-
#Cool in waterbath to 50-60 degrees
 
-
#Add antibiotics and swirl: (for 500mL of LB Agar)
 
-
#*500μL Amp stock (Amp stock = 50mg/ml)
 
-
#*1mL Kan stock (Kan stock = 10mg/ml)
 
-
#*750μL Gent stock
 
-
#*500μL Tet stock
 
-
#*500μL Chl stock
 
-
#Pour, let stand, label, store in fridge
 

Current revision

Personal tools