Skatebro:Pouring Plates

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[[Skatebro:Project08]]
 
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==Tips==
 
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*Don't forget to label
 
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*Doublecheck that antibiotics are in stock
 
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==Steps==
 
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#Set water bath to 50 degrees
 
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#Obtain 500 ml 1.2% LB agar from the media room
 
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#Melt LB in microwave:
 
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#*loosen the cap a lot, lay down paper towels in case of spill, use 50% power (enter time, press Power, 5, Start)
 
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#*monitor as you melt, takes approx. 10 minutes per bottle (if doing 2 or 3 bottles simultaneously, add 5 minutes)
 
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#Cool in waterbath to 50-60 degrees
 
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#Add antibiotics and swirl: (for 500mL of LB Agar)
 
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#*500μL Amp stock (Amp stock = 50mg/ml)
 
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#*1mL Kan stock (Kan stock = 10mg/ml)
 
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#*750μL Gent stock
 
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#*500μL Tet stock
 
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#*500μL Chl stock
 
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#Pour, let stand, label, store in fridge
 

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