Skatebro:Primer Design: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
No edit summary
(Removing all content from page)
 
(4 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Skatebro:Notebook08]]


==Tips==
*In general, want highest GC content possible
*Insert a double stop codon (taa taa)
*Binding length of 18-22 bps is ideal plus tails
*Melt temps. of forward and reverse primers should be within ~2c and ~50c is desirable

Latest revision as of 03:08, 9 February 2008