Skatebro:Primer Design: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(New page: ===Primer design=== ==Tips== want highest GC content possible insert a double stop codon (taa taa) binding length of 18-22 bps is ideal plus tails melt temps. of forward and reverse primer...) |
No edit summary |
||
| Line 1: | Line 1: | ||
==Tips== | ==Tips== | ||
want highest GC content possible | *In general, want highest GC content possible | ||
*Insert a double stop codon (taa taa) | |||
*Binding length of 18-22 bps is ideal plus tails | |||
*Melt temps. of forward and reverse primers should be within ~2c and ~50c is desirable | |||
Revision as of 19:59, 31 January 2008
Tips
- In general, want highest GC content possible
- Insert a double stop codon (taa taa)
- Binding length of 18-22 bps is ideal plus tails
- Melt temps. of forward and reverse primers should be within ~2c and ~50c is desirable
