Skatebro:Primer Design: Difference between revisions
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==Tips== | ==Tips== | ||
*In general, want highest GC content possible | *In general, want highest GC content possible | ||
Revision as of 20:01, 31 January 2008
Tips
- In general, want highest GC content possible
- Insert a double stop codon (taa taa)
- Binding length of 18-22 bps is ideal plus tails
- Melt temps. of forward and reverse primers should be within ~2c and ~50c is desirable
