Skatebro:Primer Design

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Tips)
Current revision (06:08, 9 February 2008) (view source)
(Removing all content from page)
 
Line 1: Line 1:
-
[[Skatebro:Project08]]
 
-
==Tips==
 
-
*In general, want high GC content, up to 50%
 
-
*Insert a double stop codon (taa taa)
 
-
*Binding length of 18-22 bps is ideal
 
-
*Melt temps. of forward and reverse primers should be within ~2c and ~50-60c is desirable
 
-
**analyze the landing sequence and the landing+tail Tm (can gradually increase annealing temp over pcr cycles if necessary)
 
-
*Make sure primer can only form short/thermodynamically unstable hairpins
 

Current revision

Personal tools