Skatebro:Project08

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[[User:Skatebro]]
 
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==Protocols==
 
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#[[Skatebro:Pouring_Plates]]
 
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#[[Skatebro:Primer_Design]]
 
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#[[Skatebro:PCR]]
 
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#[[Skatebro:Digestion]]
 
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#[[Skatebro:Ligation]]
 
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#[[Skatebro:Transformation]]
 
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#[[Skatebro:Electrophoresis_Gels]]
 
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#[[Skatebro:Sequencing_Prep]]
 
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==Notes==
 
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*Papers: [[Skatebro:Notes_Papers]]
 
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*Presentations: [[Skatebro:Notes_Presentations]]
 
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*Other: [[Skatebro:Notes_Other]]
 
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==Tools==
 
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*[http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=search&db=pubmed pubmed]
 
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*[http://www.neb.com/nebecomm/default.asp New England BioLabs]
 
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*[http://www.ncbi.nlm.nih.gov/blast/Blast.cgi BLAST]
 
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*[http://parts.mit.edu/registry/index.php/Main_Page Registry Main Page]
 
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*[http://slam.bs.jhmi.edu/cgi-bin/gd/gdRandDNA.cgi random DNA sequence generator]
 
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*[http://slam.bs.jhmi.edu/cgi-bin/gd/gdRevTrans.cgi reverse translate protein -> dna]
 
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*[http://slam.bs.jhmi.edu/cgi-bin/gd/gdRevTrans.cgi design a codon optimized gene]
 
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*[[Non-functional DNA sequences]]
 
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*[http://www.idtdna.com/analyzer/Applications/DilutionCalc/ stock dilution calculator]
 
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*[http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/ IDT oligoanalyzer]
 
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*[http://www.neb.com/nebecomm/tech_reference/general_data/genetic_code.asp the genetic code]
 
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*[http://tools.neb.com/NEBcutter2/index.php NEBcutter RE analyzer]
 
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*[http://www.thelabrat.com/restriction/index.shtml RE compatability]
 
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*[http://ecocyc.org/ ecocyc e. coli metabolic pathways]
 
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*[http://cgsc.biology.yale.edu/index.php CGSC coli genetic stock center]
 
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*[http://river.mit.edu/mitlibweb/FMPro?-db=RS_Items.fp5&-Lay=web&-format=ro_search.htm&-findany Vera MIT journal reserve search]
 
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*[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/cleavage_olignucleotides.asp  recommended RE cleavage overhangs]
 
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*[http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/buffer_activity_restriction_enzymes.asp? NEB buffers]
 
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*[http://www.neb.com/nebecomm/products/productF-530.asp phusion dna polymerase manual]
 

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