Skatebro:research prop: Difference between revisions
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== Project Overview == | == Project Overview == | ||
#There is much evidence to support that, under normal cellular conditions, Sus1 plays a key role in regulating SAGA activated genes by directing/confining them to the nuclear periphery for their preferential processing and export. We propose a study to visualize the transcription and motility of SAGA-dependent genes HXT1, GAL1, INO1 in sus1Δ yeast under different cellular conditions, such as high temperature, glucose starvation, and galactose induction. We would also like to | #There is much evidence to support that, under normal cellular conditions, Sus1 plays a key role in regulating SAGA activated genes by directing/confining them to the nuclear periphery for their preferential processing and export. We propose a study to visualize the transcription and motility of SAGA-dependent genes HXT1, GAL1, and INO1 in wild-type and sus1Δ yeast under different cellular conditions, such as high temperature, glucose starvation, and galactose induction. We would also like to compare genome-wide transcription levels in sus1Δ yeast in comparison to wild-type using DNA microarray for each proposed cellular condition. | ||
== Background Information == | == Background Information == | ||
Revision as of 21:13, 3 May 2007
Project Overview
- There is much evidence to support that, under normal cellular conditions, Sus1 plays a key role in regulating SAGA activated genes by directing/confining them to the nuclear periphery for their preferential processing and export. We propose a study to visualize the transcription and motility of SAGA-dependent genes HXT1, GAL1, and INO1 in wild-type and sus1Δ yeast under different cellular conditions, such as high temperature, glucose starvation, and galactose induction. We would also like to compare genome-wide transcription levels in sus1Δ yeast in comparison to wild-type using DNA microarray for each proposed cellular condition.
Background Information
Research Problem and Goals
Project Details and Methods
- Controls to do:
Predicted Outcomes
- If all goes well:
Resources
References
- Cabal G., Genovesio, A., Rodriguez-Navarro S., Zimmer C., Gadal O., Lesne A., Buc H., Feuerbach-Fournier F., Olivo-Martin J., Hurt E., Nehrbass U. SAGA interacting factors confine sub-diffusion of transcribed genes to the nuclear envelope. Nature. 2006;441(8):770-773
