Skatebro:research prop: Difference between revisions
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== Project Overview == | == Project Overview == | ||
#There is much evidence to support that, under normal cellular conditions, Sus1 plays a key role in regulating SAGA activated genes by directing/confining them to the nuclear periphery for their preferential processing and export. We propose a study using dynamic 3D tracking in live yeast cells to visualize the | #There is much evidence to support that, under normal cellular conditions, Sus1 plays a key role in regulating SAGA activated genes by directing/confining them to the nuclear periphery for their preferential processing and export. We propose a study using dynamic 3D tracking in live yeast cells to visualize the motility of SAGA-dependent genes HXT1, GAL1, and INO1 in wild-type and sus1Δ yeast under different cellular conditions, such as high temperature, glucose starvation, and galactose induction. We would also like to, for each proposed cellular condition, look at the transcription levels of these specific SAGA-dependent genes using RNA-fluorescence in situ hybridization (FISH) as well as analyze the genome-wide transcription levels in sus1Δ yeast in comparison to wild-type using DNA microarray. | ||
== Background Information == | == Background Information == | ||
Revision as of 21:19, 3 May 2007
Project Overview
- There is much evidence to support that, under normal cellular conditions, Sus1 plays a key role in regulating SAGA activated genes by directing/confining them to the nuclear periphery for their preferential processing and export. We propose a study using dynamic 3D tracking in live yeast cells to visualize the motility of SAGA-dependent genes HXT1, GAL1, and INO1 in wild-type and sus1Δ yeast under different cellular conditions, such as high temperature, glucose starvation, and galactose induction. We would also like to, for each proposed cellular condition, look at the transcription levels of these specific SAGA-dependent genes using RNA-fluorescence in situ hybridization (FISH) as well as analyze the genome-wide transcription levels in sus1Δ yeast in comparison to wild-type using DNA microarray.
Background Information
Research Problem and Goals
Project Details and Methods
- Controls to do:
Predicted Outcomes
- If all goes well:
Resources
References
- Cabal G., Genovesio, A., Rodriguez-Navarro S., Zimmer C., Gadal O., Lesne A., Buc H., Feuerbach-Fournier F., Olivo-Martin J., Hurt E., Nehrbass U. SAGA interacting factors confine sub-diffusion of transcribed genes to the nuclear envelope. Nature. 2006;441(8):770-773
