Skatebro:research prop: Difference between revisions
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== Project Overview == | == Project Overview == | ||
There is much evidence to support that, under normal cellular conditions, Sus1 plays a key role in regulating SAGA activated genes by directing/confining them to the nuclear periphery for their preferential processing and export. We propose a 3-part study to look at changes in gene motility and transcription in wild-type and sus1Δ yeast under different cellular conditions, such as high temperature, glucose starvation, and galactose induction. | There is much evidence to support that, under normal cellular conditions, Sus1 plays a key role in regulating SAGA activated genes by directing/confining them to the nuclear periphery for their preferential processing and export. We propose a 3-part study to look at changes in gene motility and transcription in wild-type and sus1Δ yeast under different cellular conditions, such as high temperature, glucose starvation, and galactose induction. | ||
*Part 1: employ dynamic 3D tracking in live cells to visualize the motility of SAGA-dependent genes HXT1, GAL1, and INO1 | *Part 1: employ dynamic 3D tracking in live yeast cells to visualize the motility of SAGA-dependent genes HXT1, GAL1, and INO1 | ||
*Part 2: use RNA-fluorescence in situ hybridization (FISH)to look at HXT1, GAL1, INO1 transcription levels | *Part 2: use RNA-fluorescence in situ hybridization (FISH)to look at HXT1, GAL1, INO1 transcription levels | ||
*Part 3: use DNA microarray to look at changes in genome-wide transcription levels in sus1Δ yeast. | *Part 3: use DNA microarray to look at changes in genome-wide transcription levels in sus1Δ yeast. | ||
Revision as of 21:44, 3 May 2007
Project Overview
There is much evidence to support that, under normal cellular conditions, Sus1 plays a key role in regulating SAGA activated genes by directing/confining them to the nuclear periphery for their preferential processing and export. We propose a 3-part study to look at changes in gene motility and transcription in wild-type and sus1Δ yeast under different cellular conditions, such as high temperature, glucose starvation, and galactose induction.
- Part 1: employ dynamic 3D tracking in live yeast cells to visualize the motility of SAGA-dependent genes HXT1, GAL1, and INO1
- Part 2: use RNA-fluorescence in situ hybridization (FISH)to look at HXT1, GAL1, INO1 transcription levels
- Part 3: use DNA microarray to look at changes in genome-wide transcription levels in sus1Δ yeast.
Background Information
Research Problem and Goals
Project Details and Methods
- Controls to do:
Predicted Outcomes
- If all goes well:
Resources
References
- Cabal G., Genovesio, A., Rodriguez-Navarro S., Zimmer C., Gadal O., Lesne A., Buc H., Feuerbach-Fournier F., Olivo-Martin J., Hurt E., Nehrbass U. SAGA interacting factors confine sub-diffusion of transcribed genes to the nuclear envelope. Nature. 2006;441(8):770-773
