Skatebro:research prop: Difference between revisions
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#Sus1 interacts with both the SAGA complex and the Sac3-Thp1-Cdc31 complex, which binds to Nuclear Pore Complexes (NPC) at the nuclear periphery. | #Sus1 interacts with both the SAGA complex and the Sac3-Thp1-Cdc31 complex, which binds to Nuclear Pore Complexes (NPC) at the nuclear periphery. | ||
#Under galactose induction, while Sus1 was found to be dispensable for GAL1 activation, Sus1 was found to be critical for gene motility and confinement of activated GAL loci to the nuclear periphery. | #Under galactose induction, while Sus1 was found to be dispensable for GAL1 activation, Sus1 was found to be critical for gene motility and confinement of activated GAL loci to the nuclear periphery. | ||
#Sus1 is hypothesized to be likely involved in transcription coupled mRNA export in a manner similar to the following schematic: | #Sus1 is hypothesized to be likely involved in transcription coupled mRNA export in a manner similar to the following schematic:[[Image:SUS1.jpg|250px|center]] | ||
[[Image:SUS1.jpg|250px|center]] | #Our Module 3 research suggests that, as temperature is increased, sus1Δ yeast grow more robustly than wild-type yeast on galactose media. | ||
#Our Module 3 research suggests that sus1Δ yeast grow more robustly than wild-type yeast on galactose media | #Intrigued by this unpredicted finding, we propose a research project aimed at studying Sus1's role in gene transcription and motility under different cellular conditions. | ||
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== Research Problem and Goals == | == Research Problem and Goals == | ||
Revision as of 22:13, 3 May 2007
Project Overview
There is much evidence to support that, under standard cellular conditions, Sus1 plays a key role in regulating SAGA activated genes by directing/confining them to the nuclear periphery for their preferential processing and export. We propose a 3-part study to look at changes in SAGA-dependent gene motility and transcription in wild-type and sus1Δ yeast under different cellular conditions, such as high temperature combined with galactose induction.
- Part 1: use dynamic 3D tracking in live yeast cells to visualize the motility of SAGA-dependent genes HXT1, GAL1, and INO1
- Part 2: use RNA-fluorescence in situ hybridization (FISH) to look at HXT1, GAL1, INO1 transcription levels
- Part 3: use DNA microarray to look at changes in genome-wide transcription levels in sus1Δ yeast.
Background Information
- SAGA complex dominates transcriptional activation at a minumum of 10% of the meausurable yeast genome, and these genes tend to be stress induced.
- Genes GAL1, HXT1, and INO1 have been previously studied and confirmed to be SAGA-dependent.
- Sus1 interacts with both the SAGA complex and the Sac3-Thp1-Cdc31 complex, which binds to Nuclear Pore Complexes (NPC) at the nuclear periphery.
- Under galactose induction, while Sus1 was found to be dispensable for GAL1 activation, Sus1 was found to be critical for gene motility and confinement of activated GAL loci to the nuclear periphery.
- Sus1 is hypothesized to be likely involved in transcription coupled mRNA export in a manner similar to the following schematic:
- Our Module 3 research suggests that, as temperature is increased, sus1Δ yeast grow more robustly than wild-type yeast on galactose media.
- Intrigued by this unpredicted finding, we propose a research project aimed at studying Sus1's role in gene transcription and motility under different cellular conditions.
Research Problem and Goals
Project Details and Methods
- Controls to do:
Predicted Outcomes
- If all goes well:
Resources
References
- Cabal G., Genovesio, A., Rodriguez-Navarro S., Zimmer C., Gadal O., Lesne A., Buc H., Feuerbach-Fournier F., Olivo-Martin J., Hurt E., Nehrbass U. SAGA interacting factors confine sub-diffusion of transcribed genes to the nuclear envelope. Nature. 2006;441(8):770-773
