Smolke:Enzyme inventory: Difference between revisions
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*Two restriction enzyme coolers are in -20C over the Quanta bay, one labeled common. | *Two restriction enzyme coolers are in -20C over the Quanta bay, one labeled common. | ||
*Only common enzymes may have backup, which are stored in main -20C. | *Only common enzymes may have backup, which are stored in main -20C. | ||
* | *Common enzymes: AgeI, AleI, ApaI, AvrII, BamHI, DpnI, EcoRI, EcoRV, HindIII, MfeI, NotI, SacI, SacII, SalI, XbaI, XhoI | ||
*If the enzyme is available in HF form, only the HF form will be in the backup unless otherwise requested. | *If the enzyme is available in HF form, only the HF form will be in the backup unless otherwise requested. | ||
*When the working stock has been | *When the working stock has been used up, replace with a new tube. | ||
*Backup ligases and CIP are also in the backup cooler in main -20C. | *Backup ligases and CIP are also in the backup cooler in main -20C. | ||
Revision as of 13:38, 9 September 2010
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Restriction Enzymes in Stocks
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