Smolke:Enzyme inventory: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(→Notes) |
|||
(43 intermediate revisions by 4 users not shown) | |||
Line 1: | Line 1: | ||
{{Smolke_Top}} | {{Smolke_Top}} | ||
== | ==Rules== | ||
*Two restriction enzyme coolers are in -20C over the Quanta bay, one labeled common. | |||
*Only common enzymes may have backup, which are stored in main -20C. | |||
*Common enzymes: AgeI, AleI, ApaI, AvrII, BamHI, DpnI, EcoRI, EcoRV, HindIII, MfeI, NotI, SacI, SacII, SalI, XbaI, XhoI | |||
*The enzymes not listed are in the non-common enzyme cooler. | |||
*If the enzyme is available in HF form, only the HF form will be in the backup unless otherwise requested. | |||
*When the working stock has been used up, replace with a new tube from the backup cooler and write the enzyme on the whiteboard. | |||
*Backup ligases and CIP are also in the backup cooler in main -20C. | |||
==Restriction Enzymes in Stocks== | |||
{| border="1" class="wikitable" style="text-align:center" | {| border="1" class="wikitable" style="text-align:center" | ||
! Enzyme !! Regular !! HF !! Regular Backup !! HF Backup | ! Enzyme !! Regular !! HF !! Regular Backup !! HF Backup | ||
|- | |- | ||
! AgeI || | ! AflII ||Y|| || || | ||
|- | |||
! AgeI ||Y||Y|| ||Y | |||
|- | |||
! AleI ||Y|| ||Y|| | |||
|- | |||
! ApaI ||Y|| ||YY|| | |||
|- | |||
! AscI ||Y|| || || | |||
|- | |||
! AsiSI ||Y|| ||Y|| | |||
|- | |||
! AvrII ||Y|| ||Y|| | |||
|- | |||
! BamHI ||Y||Y|| ||Y | |||
|- | |||
! BglII ||Y|| || || | |||
|- | |||
! BsaI || ||Y|| || | |||
|- | |||
! BsrGI ||Y|| || || | |||
|- | |||
! BstBI ||Y|| || || | |||
|- | |||
! BstXI ||Y|| || || | |||
|- | |||
! Bsu36I ||Y|| || || | |||
|- | |||
! ClaI ||Y|| ||Y|| | |||
|- | |||
! DpnI ||Y|| ||Y|| | |||
|- | |||
! EcoRI || ||Y|| ||Y | |||
|- | |||
! EcoRV || ||Y|| ||Y | |||
|- | |||
! FseI ||Jay's -80|| || || | |||
|- | |||
! HindIII ||Y||Y||Y|| | |||
|- | |||
! KasI || || || ||Y | |||
|- | |||
! KpnI ||Y||Y|| ||Y | |||
|- | |||
! MfeI || ||Y|| ||Y | |||
|- | |||
! NdeI ||Y|| || || | |||
|- | |||
! NheI ||Y||Y|| || | |||
|- | |||
! NotI ||Y||Y|| ||Y | |||
|- | |||
! NruI ||Y|| || || | |||
|- | |||
! PacI ||Y|| || || | |||
|- | |- | ||
! | ! PmeI ||Y|| || || | ||
|- | |- | ||
! | ! PstI ||Y|| || || | ||
|- | |- | ||
! | ! PvuII || || Y || || | ||
|- | |- | ||
! | ! SacI || ||Y|| ||Y | ||
|- | |- | ||
! | ! SacII ||Y|| ||Y|| | ||
|- | |- | ||
! | ! SalI ||Y||Y|| ||Y | ||
|- | |- | ||
! | ! SapI ||Y|| || || | ||
|- | |- | ||
! | ! SbfI || ||Y|| || | ||
|- | |- | ||
! | ! SpeI ||Y|| ||Y|| | ||
|- | |- | ||
! | ! SphI || ||Y|| || | ||
|- | |- | ||
! | ! SwaI ||Y|| || || | ||
|- | |- | ||
! | ! XbaI ||Y|| ||Y|| | ||
|- | |- | ||
! | ! XhoI ||Y|| ||Y|| | ||
|- | |- | ||
! | ! XmaI ||Y|| || || | ||
|- | |- | ||
|} | |} | ||
== | ==Useful Links== | ||
* | *What is [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/HF_enzymes.asp high fidelity (HF)] restriction enzyme? | ||
* | *[http://www.neb.com/nebecomm/DoubleDigestCalculator.asp Double digest] finder | ||
==Contact== | ==Contact== | ||
*remus |
Latest revision as of 14:40, 21 August 2014
Rules
Restriction Enzymes in Stocks
Useful Links
Contact
|