Smolke:Enzyme inventory: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(31 intermediate revisions by 4 users not shown) | |||
Line 2: | Line 2: | ||
==Rules== | ==Rules== | ||
*Two enzyme coolers are in -20C over the Quanta bay. | *Two restriction enzyme coolers are in -20C over the Quanta bay, one labeled common. | ||
*The | *Only common enzymes may have backup, which are stored in main -20C. | ||
*If the enzyme is available in HF form, only the HF form will be | *Common enzymes: AgeI, AleI, ApaI, AvrII, BamHI, DpnI, EcoRI, EcoRV, HindIII, MfeI, NotI, SacI, SacII, SalI, XbaI, XhoI | ||
*When the working stock has been | *The enzymes not listed are in the non-common enzyme cooler. | ||
*If the enzyme is available in HF form, only the HF form will be in the backup unless otherwise requested. | |||
*When the working stock has been used up, replace with a new tube from the backup cooler and write the enzyme on the whiteboard. | |||
*Backup ligases and CIP are also in the backup cooler in main -20C. | |||
==Restriction Enzymes in Stocks== | ==Restriction Enzymes in Stocks== | ||
Line 11: | Line 14: | ||
! Enzyme !! Regular !! HF !! Regular Backup !! HF Backup | ! Enzyme !! Regular !! HF !! Regular Backup !! HF Backup | ||
|- | |- | ||
! | ! AflII ||Y|| || || | ||
|- | |- | ||
! | ! AgeI ||Y||Y|| ||Y | ||
|- | |- | ||
! AleI ||Y|| ||Y|| | ! AleI ||Y|| ||Y|| | ||
|- | |- | ||
! ApaI ||Y|| || | ! ApaI ||Y|| ||YY|| | ||
|- | |- | ||
! AscI ||Y|| || || | ! AscI ||Y|| || || | ||
|- | |- | ||
! AsiSI ||Y | ! AsiSI ||Y|| ||Y|| | ||
|- | |||
! AvrII ||Y|| ||Y|| | |||
|- | |- | ||
! BamHI ||Y||Y|| ||Y | ! BamHI ||Y||Y|| ||Y | ||
Line 32: | Line 37: | ||
|- | |- | ||
! BstBI ||Y|| || || | ! BstBI ||Y|| || || | ||
|- | |||
! BstXI ||Y|| || || | |||
|- | |- | ||
! Bsu36I ||Y|| || || | ! Bsu36I ||Y|| || || | ||
|- | |||
! ClaI ||Y|| ||Y|| | |||
|- | |- | ||
! DpnI ||Y|| ||Y|| | ! DpnI ||Y|| ||Y|| | ||
|- | |- | ||
! EcoRI || ||Y|| ||Y | |||
! EcoRI || | |||
|- | |- | ||
! EcoRV || | ! EcoRV || ||Y|| ||Y | ||
|- | |- | ||
! | ! FseI ||Jay's -80|| || || | ||
|- | |- | ||
! | ! HindIII ||Y||Y||Y|| | ||
|- | |- | ||
! | ! KasI || || || ||Y | ||
|- | |- | ||
! | ! KpnI ||Y||Y|| ||Y | ||
|- | |- | ||
! | ! MfeI || ||Y|| ||Y | ||
|- | |- | ||
! | ! NdeI ||Y|| || || | ||
|- | |- | ||
! | ! NheI ||Y||Y|| || | ||
|- | |- | ||
! NotI ||Y||Y|| ||Y | ! NotI ||Y||Y|| ||Y | ||
|- | |- | ||
! NruI ||Y|| || || | ! NruI ||Y|| || || | ||
|- | |- | ||
! PacI ||Y|| || || | ! PacI ||Y|| || || | ||
Line 73: | Line 72: | ||
! PmeI ||Y|| || || | ! PmeI ||Y|| || || | ||
|- | |- | ||
! | ! PstI ||Y|| || || | ||
|- | |- | ||
! | ! PvuII || || Y || || | ||
|- | |- | ||
! SacI || ||Y|| ||Y | ! SacI || ||Y|| ||Y | ||
|- | |- | ||
! SacII || Y || ||Y|| | ! SacII ||Y|| ||Y|| | ||
|- | |- | ||
! | ! SalI ||Y||Y|| ||Y | ||
|- | |- | ||
! SapI ||Y|| || || | ! SapI ||Y|| || || | ||
|- | |- | ||
! SbfI ||Y|| || || | ! SbfI || ||Y|| || | ||
|- | |||
! SpeI ||Y|| ||Y|| | |||
|- | |- | ||
! | ! SphI || ||Y|| || | ||
|- | |- | ||
! | ! SwaI ||Y|| || || | ||
|- | |- | ||
! XbaI ||Y|| ||Y|| | ! XbaI ||Y|| ||Y|| | ||
Line 104: | Line 105: | ||
==Contact== | ==Contact== | ||
*remus |
Latest revision as of 14:40, 21 August 2014
Rules
Restriction Enzymes in Stocks
Useful Links
Contact
|