Smolke:Enzyme inventory: Difference between revisions
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*Only common enzymes may have backup, which are stored in main -20C. | *Only common enzymes may have backup, which are stored in main -20C. | ||
*Common enzymes: AgeI, AleI, ApaI, AvrII, BamHI, DpnI, EcoRI, EcoRV, HindIII, MfeI, NotI, SacI, SacII, SalI, XbaI, XhoI | *Common enzymes: AgeI, AleI, ApaI, AvrII, BamHI, DpnI, EcoRI, EcoRV, HindIII, MfeI, NotI, SacI, SacII, SalI, XbaI, XhoI | ||
*The enzymes not listed are in the non-common enzyme cooler. | |||
*If the enzyme is available in HF form, only the HF form will be in the backup unless otherwise requested. | *If the enzyme is available in HF form, only the HF form will be in the backup unless otherwise requested. | ||
*When the working stock has been used up, replace with a new tube. | *When the working stock has been used up, replace with a new tube from the backup cooler and write the enzyme on the whiteboard. | ||
*Backup ligases and CIP are also in the backup cooler in main -20C. | *Backup ligases and CIP are also in the backup cooler in main -20C. | ||
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! AsiSI ||Y|| ||Y|| | ! AsiSI ||Y|| ||Y|| | ||
|- | |- | ||
! AvrII ||Y|| || | ! AvrII ||Y|| ||Y|| | ||
|- | |- | ||
! BamHI ||Y||Y|| ||Y | ! BamHI ||Y||Y|| ||Y | ||
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|- | |- | ||
! HindIII ||Y||Y||Y|| | ! HindIII ||Y||Y||Y|| | ||
|- | |||
! KasI || || || ||Y | |||
|- | |- | ||
! KpnI ||Y||Y|| ||Y | ! KpnI ||Y||Y|| ||Y | ||
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==Contact== | ==Contact== | ||
*remus |
Latest revision as of 14:40, 21 August 2014
Rules
Restriction Enzymes in Stocks
Useful Links
Contact
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