Smolke:Enzyme inventory

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(Restriction Enzymes in Stocks)
(Rules)
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*Only common enzymes may have backup, which are stored in main -20C.
*Only common enzymes may have backup, which are stored in main -20C.
*Common enzymes: AgeI, AleI, ApaI, AvrII, BamHI, DpnI, EcoRI, EcoRV, HindIII, MfeI, NotI, SacI, SacII, SalI, XbaI, XhoI   
*Common enzymes: AgeI, AleI, ApaI, AvrII, BamHI, DpnI, EcoRI, EcoRV, HindIII, MfeI, NotI, SacI, SacII, SalI, XbaI, XhoI   
 +
*The enzymes not listed are in the non-common enzyme cooler.
*If the enzyme is available in HF form, only the HF form will be in the backup unless otherwise requested.
*If the enzyme is available in HF form, only the HF form will be in the backup unless otherwise requested.
-
*When the working stock has been used up, replace with a new tube.
+
*When the working stock has been used up, replace with a new tube from the backup cooler and write the enzyme on the whiteboard.
*Backup ligases and CIP are also in the backup cooler in main -20C.
*Backup ligases and CIP are also in the backup cooler in main -20C.

Revision as of 20:37, 15 February 2012

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Contents

Rules

  • Two restriction enzyme coolers are in -20C over the Quanta bay, one labeled common.
  • Only common enzymes may have backup, which are stored in main -20C.
  • Common enzymes: AgeI, AleI, ApaI, AvrII, BamHI, DpnI, EcoRI, EcoRV, HindIII, MfeI, NotI, SacI, SacII, SalI, XbaI, XhoI
  • The enzymes not listed are in the non-common enzyme cooler.
  • If the enzyme is available in HF form, only the HF form will be in the backup unless otherwise requested.
  • When the working stock has been used up, replace with a new tube from the backup cooler and write the enzyme on the whiteboard.
  • Backup ligases and CIP are also in the backup cooler in main -20C.

Restriction Enzymes in Stocks

Enzyme Regular HF Regular Backup HF Backup
AflII Y
AgeI YY Y
AleI Y Y
ApaI Y YY
AscI Y
AsiSI Y Y
AvrII Y YY
BamHI YY Y
BglII Y
BsaI Y
BsrGI Y
BstBI Y
BstXI Y
Bsu36I Y
ClaI Y Y
DpnI Y Y
EcoRI Y Y
EcoRV Y Y
FseI Jay's -80
HindIII YYY
KpnI YY Y
MfeI Y Y
NdeI Y
NheI YY
NotI YY Y
NruI Y
PacI Y
PmeI Y
PstI Y
PvuII Y
SacI Y Y
SacII Y Y
SalI YY Y
SapI Y
SbfI Y
SpeI Y Y
SphI Y
SwaI Y
XbaI Y Y
XhoI Y Y
XmaI Y

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