Smolke:Enzyme inventory

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Rules

  • Two enzyme coolers are in -20C over the Quanta bay. One with a green tape on top stores the commonly used enzymes while the other stores infrequently used enzymes.
  • The backup cooler of the commonly used enzymes (including polymerases and ligases) is in main -20C.
  • If the enzyme is available in HF form, only the HF form will be backed up unless otherwise requested.
  • When the working stock has been (or almost) used up, replace (or combine) with a new tube.

Restriction Enzymes in Stocks

Enzyme Regular HF Regular Backup HF Backup
AgeI Y Y
AleI Y Y
ApaI Y Y
BamHI Y Y Y
DpnI Y Y
EcoRI Y Y Y
EcoRV Y Y Y
HindIII Y Y
MfeI Y Y
NotI Y Y Y
SacI Y Y
SacII Y Y
SaII Y Y Y
XbaI Y Y
XhoI Y Y

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