Rules
- Two restriction enzyme coolers are in -20C over the Quanta bay, one labeled common.
- Only common enzymes have backup, which are stored in main -20C.
- If the enzyme is available in HF form, only the HF form will be in the backup unless otherwise requested.
- When the working stock has been (or almost) used up, replace (or combine) with a new tube.
- Backup ligases and CIP are also in the backup cooler in main -20C.
Restriction Enzymes in Stocks
Enzyme |
Regular |
HF |
Regular Backup |
HF Backup
|
AgeI |
|
Y |
|
Y
|
AhdI |
Y |
|
|
|
AleI |
Y |
|
Y |
|
ApaI |
Y |
|
Y |
|
AscI |
Y |
|
|
|
AsiSI |
Y (x2) |
|
|
|
BamHI |
Y |
Y |
|
Y
|
BglII |
Y |
|
|
|
BsaI |
|
Y |
|
|
BsrGI |
Y |
|
|
|
BstBI |
Y |
|
|
|
Bsu36I |
Y |
|
|
|
DpnI |
Y |
|
Y |
|
EagI |
Y |
|
|
|
EcoRI |
Y |
Y |
|
Y
|
EcoRV |
Y |
Y |
|
Y
|
HindIII |
Y |
|
Y |
|
HpaI |
Y |
|
|
|
KpnI |
Y |
|
Y |
|
MfeI |
|
Y |
|
Y
|
MluI |
Y |
|
|
|
MscI |
Y |
|
|
|
MspA1I |
Y |
|
|
|
NaeI |
Y |
|
|
|
NdeI |
Y |
|
|
|
NheI |
Y |
|
|
|
NotI |
Y |
Y |
|
Y
|
NruI |
Y |
|
|
|
NsiI |
Y |
|
|
|
PacI |
Y |
|
|
|
PmeI |
Y |
|
|
|
PmlI |
Y |
|
|
|
PstI |
Y |
|
|
|
SacI |
|
Y |
|
Y
|
SacII |
Y |
|
Y |
|
SaII |
Y |
Y |
|
Y
|
SapI |
Y |
|
|
|
SbfI |
Y |
|
|
|
SpeI |
Y |
|
|
|
SphI |
Y |
|
|
|
XbaI |
Y |
|
Y |
|
XhoI |
Y |
|
Y |
|
XmaI |
Y |
|
|
|
Useful Links
Contact
Joe
|