Smolke:Lab Jobs

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Laboratory Jobs and Administrative Assignments Andrew YNB (I), SOC computer administrator and databases (back up) Andy plasmid prep buffers (P1, P2, N3, PE, EB, stocks), aliquot dNTPs (II) freezer(-80/-20) Arwen LB (I), pH meters, aliquot dNTPs (I) CE Christina general lab ordering Drew electrocompetent cells (I), water baths 4 refrigerator/cold room organization Elizabeth glass beads, toothpicks, used plate sterilization, glass/plastic washing sterilization, disposable sterilization safety officer, general lab organization/maintenance, cleaning general areas, order inventory, inventory MSDS sheets, organize library Isis LB plates (II) databases and software, secondary lab safety officer Jay 10x sugar stocks (dextrose), TAE stocks, 60% glycerol, sodium acetate, EDTA stocks TC Room, cryogenic storage LN2 refill (I) Joe amino acid stocks, 10x drop out stocks for plates general enzyme ordering and information Josh -UT plates, LB (II), antibiotics lab wiki, inventory chemical waste bottles/containers (fume hood), sharps Kate -U plates, YNB (II) general enzyme ordering and information (back up) Kathy electrocompetent cells (III), general cleaning of high speed centrifuge Quanta (back up), computer administrator (back up) Leo LB plates (I) radioactivity safety officer Michael -T plates, YPD plates, YPD LC-MS Ryan LB plates (III), sterile water cryogenic storage LN2 refill (II) Yvonne electrocompetent cells (II) computer administrator, Quanta Notes General lab courtesy - if you use a whole sleeve of plates, make another. If you use a lot of a stock or finish it, make some more. Everyone should help aliquot electrocomp cells. Solution I and Solution II are now the responsibility of those who use them regularly. TBE is also the responsibility of those who are using it regularly. How It Works Each person is assigned a laboratory job (or set of jobs based on intensity of the assigned job) and an administrative role (indicated in italics). Please keep up with your assigned jobs. Note, that it is everyone's responsibility to load tips, load the dishwasher, and rinse out your glassware. Also, while general DO plates are made (-U, -T, and -UT) as common stocks, all other DO plates are made by the individual using them.