Smolke:Protocols

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Note: If possible, please follow the standard protocol format. Protocols Cloning Primer Design Plasmid prep with Epoch columns and homemade buffers PCR amplifying inserts from plasmids When to use phenol-chloroform vs. columns Restriction digests Gel extraction Ligation Transformation Colony PCR Screening by digestion Sequencing with Elim Making freezer stocks Gene assembly Yeast Protocols Pouring yeast dropout plates Yeast plasmid transformation Yeast colony PCR Anti-V5 Western Library-scale yeast transformations Yeast chromosomal modifications Yeast plasmid prep Yeast metabolite extraction Observations and Discussion CSY3 Tissue Culture room Adherent cell care and feeding Media stocks Freezer stock management and database General maintenance General sterility Quanta Preventing Clogs Clearing Quanta Clogs Miscellaneous Glass Beads Poster Printing My pipet-aid is broken! Cleaning Bottles for the LC Solutions Pfu Buffer Taq Buffer 10x DO Media dNTP Mixture 5-Fluoroorotic acid Antibiotic Stocks