Smolke:Protocols

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(Cloning)
(Yeast)
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===Cloning===
===Cloning===
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:*[[Smolke:Protocols/Primer Design|Primer Design]]
:*[[Smolke:Protocols/Plasmid prep|Plasmid prep with Epoch columns and homemade buffers]]
:*[[Smolke:Protocols/Plasmid prep|Plasmid prep with Epoch columns and homemade buffers]]
:*[[Smolke:Protocols/Insert prep|PCR amplifying inserts from plasmids]]
:*[[Smolke:Protocols/Insert prep|PCR amplifying inserts from plasmids]]
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:*[[Smolke:Protocols/Gel extraction|Gel extraction]]
:*[[Smolke:Protocols/Gel extraction|Gel extraction]]
:*[[Smolke:Protocols/Ligation|Ligation]]
:*[[Smolke:Protocols/Ligation|Ligation]]
-
:*[[Smolke:Protocols/Transformation|Transformation]]
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:*[[Smolke:Protocols/Transformation|Electrocompetent Transformation]]
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:*[[Smolke:Protocols/Heat Shock Transformation|Heat Shock Transformation]]
:*[[Smolke:Protocols/Screening|Colony PCR]]
:*[[Smolke:Protocols/Screening|Colony PCR]]
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:*[[Smolke:Protocols/Digest screening|Screening by digestion]]
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:*[[Smolke:Protocols/Digest Screening|Screening by digestion]]
:*[[Smolke:Protocols/Sequencing|Sequencing with Elim]]
:*[[Smolke:Protocols/Sequencing|Sequencing with Elim]]
:*[[Smolke:Protocols/Freezer stocks|Making freezer stocks]]
:*[[Smolke:Protocols/Freezer stocks|Making freezer stocks]]
 +
:*[[Smolke:Protocols/Gene assembly|Gene assembly]]
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:*[[Smolke:Protocols/EtBr stock|EtBr stock]]
===Yeast===
===Yeast===
:'''Protocols'''
:'''Protocols'''
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:*[[Smolke:Dropout plates|Pouring yeast dropout plates]]
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:*[[Smolke:Protocols/Yeast Media Recipes | Yeast Media Recipes]]
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:*[[Smolke:Colony_PCR_tsb|Colony PCR (Travis' version)]]
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:*[[Smolke:Protocols/Dropout plates|Pouring yeast dropout plates]]
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:*[[Smolke:Western|Anti-V5 Western]]
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:*[[Smolke:Protocols/Yeast transformation|Yeast plasmid transformation]]
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:*[[Smolke:Library_transformation|Library-scale yeast transformations]]
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:*[[Smolke:Protocols/Quick yeast transformation|Quick Yeast plasmid transformation]]
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:*[[Smolke:Protocols/Yeast Colony PCR|Yeast colony PCR]]
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:*[[Smolke:Protocols/Western|Anti-V5 Western]]
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:*[[Smolke:Protocols/Library_transformation|Library-scale yeast transformations & Gap repair]]
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:*[[Smolke:Protocols/Chromosomal Modification|Yeast chromosomal modifications]]
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:*[[Smolke:Protocols/Yeast plasmid prep|Yeast plasmid prep]]
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:*[[Smolke:Protocols/Feeding yeast cultures|Feeding yeast cultures]]
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:*[[Smolke:Protocols/Yeast metabolite extraction|Yeast metabolite extraction]]
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:*[[Smolke:Protocols/Heme measurements|Heme measurements]]
:'''Observations and Discussion'''
:'''Observations and Discussion'''
:*[http://wiki.yeastgenome.org/index.php/CommunityW303.html CSY3]
:*[http://wiki.yeastgenome.org/index.php/CommunityW303.html CSY3]
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===DNA===
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===Tissue Culture room===
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:'''Protocols'''
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:*[[Smolke:Protocols/Adherent cell care and feeding|Adherent cell care and feeding]]
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:*[[Smolke:Oligos | Oligos]]
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:*[[Smolke:Protocols/Media stocks|Media stocks]]
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:*[[Smolke:Protocols/Freezer stock management and database|Freezer stock management and database]]
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:*[[Smolke:Protocols/General Maintenance|General maintenance]]
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:*[[Smolke:Protocols/General Sterility|General sterility]]
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===Flow Cytometer===
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===Quanta===
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:'''Protocols'''
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:*[[Smolke:Protocols/Preventing Clogs|Preventing Clogs]]
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:*[[Despaux:Ma_FlowCyCompensation|Compensation]]
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:*[[Smolke:Protocols/Clearing Clogs|Clearing Clogs]]
 +
 
 +
===VYB===
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:*[[Smolke:Protocols/Guidelines|Guidelines]]
===Miscellaneous===
===Miscellaneous===
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:'''Protocols'''
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:*[[Smolke:Protocols/Glass Beads|Glass Beads]]
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:*[[Smolke:Coffee|Making coffee]]
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:*[[Smolke:Protocols/Printing Posters|Poster Printing]]
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:*[[Smolke:Lab drinks|Lab drinks]]
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:*[[Smolke:Protocols/Pipet-Aid|My pipet-aid is broken!]]
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:*[[Smolke:Dishwasher|Dishwasher]]
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:*[[Smolke:Protocols/Cleaning Bottles for the LC|Cleaning Bottles for the LC]]
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:*[[Smolke:Glass Beads|Glass Beads]]
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-
 
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-
===Mammalian===
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-
 
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===Bacteria===
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==Solutions==
==Solutions==
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:'''Recipes'''
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*[[Smolke:Protocols/Pfu Buffer|Pfu Buffer]]
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:*[[Smolke:Pfu Buffer|Pfu Buffer]]
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*[[Smolke:Protocols/Taq Buffer|Taq Buffer]]
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:*[[Smolke:Taq Buffer|Taq Buffer]]
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*[[Smolke:Protocols/DO Media|10x DO Media]]
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:*[[Smolke:DO Media|10x DO Media]]
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*[[Smolke:Protocols/dNTP Mixture|dNTP Mixture]]
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:*[[Smolke:dNTP Mixture|dNTP Mixture]]
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*[[Smolke:Protocols/5FOA|5-Fluoroorotic acid]]
 +
*[[Smolke:Protocols/Antibiotics|Antibiotic Stocks]]

Revision as of 15:30, 16 October 2012

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Note: If possible, please follow the standard protocol format.

Contents

Protocols

Cloning

Yeast

Protocols
Observations and Discussion

Tissue Culture room

Quanta

VYB

Miscellaneous

Solutions

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