Smolke:Protocols
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:*[[Smolke:Protocols/Gel extraction|Gel extraction]] | :*[[Smolke:Protocols/Gel extraction|Gel extraction]] | ||
:*[[Smolke:Protocols/Ligation|Ligation]] | :*[[Smolke:Protocols/Ligation|Ligation]] | ||
| - | :*[[Smolke:Protocols/Transformation|Transformation]] | + | :*[[Smolke:Protocols/Transformation|Electrocompetent Transformation]] |
| + | :*[[Smolke:Protocols/Heat Shock Transformation|Heat Shock Transformation]] | ||
:*[[Smolke:Protocols/Screening|Colony PCR]] | :*[[Smolke:Protocols/Screening|Colony PCR]] | ||
:*[[Smolke:Protocols/Digest Screening|Screening by digestion]] | :*[[Smolke:Protocols/Digest Screening|Screening by digestion]] | ||
:*[[Smolke:Protocols/Sequencing|Sequencing with Elim]] | :*[[Smolke:Protocols/Sequencing|Sequencing with Elim]] | ||
:*[[Smolke:Protocols/Freezer stocks|Making freezer stocks]] | :*[[Smolke:Protocols/Freezer stocks|Making freezer stocks]] | ||
| + | :*[[Smolke:Protocols/Gene assembly|Gene assembly]] | ||
| + | :*[[Smolke:Protocols/EtBr stock|EtBr stock]] | ||
===Yeast=== | ===Yeast=== | ||
:'''Protocols''' | :'''Protocols''' | ||
| + | :*[[Smolke:Protocols/Yeast Media Recipes | Yeast Media Recipes]] | ||
:*[[Smolke:Protocols/Dropout plates|Pouring yeast dropout plates]] | :*[[Smolke:Protocols/Dropout plates|Pouring yeast dropout plates]] | ||
:*[[Smolke:Protocols/Yeast transformation|Yeast plasmid transformation]] | :*[[Smolke:Protocols/Yeast transformation|Yeast plasmid transformation]] | ||
| - | :*[[Smolke:Protocols/Yeast Colony PCR|Yeast | + | :*[[Smolke:Protocols/Quick yeast transformation|Quick Yeast plasmid transformation]] |
| + | :*[[Smolke:Protocols/Yeast Colony PCR|Yeast colony PCR]] | ||
:*[[Smolke:Protocols/Western|Anti-V5 Western]] | :*[[Smolke:Protocols/Western|Anti-V5 Western]] | ||
| - | :*[[Smolke:Protocols/Library_transformation|Library-scale yeast transformations]] | + | :*[[Smolke:Protocols/Library_transformation|Library-scale yeast transformations & Gap repair]] |
:*[[Smolke:Protocols/Chromosomal Modification|Yeast chromosomal modifications]] | :*[[Smolke:Protocols/Chromosomal Modification|Yeast chromosomal modifications]] | ||
| + | :*[[Smolke:Protocols/Yeast plasmid prep|Yeast plasmid prep]] | ||
| + | :*[[Smolke:Protocols/Feeding yeast cultures|Feeding yeast cultures]] | ||
| + | :*[[Smolke:Protocols/Yeast metabolite extraction|Yeast metabolite extraction]] | ||
| + | :*[[Smolke:Protocols/Heme measurements|Heme measurements]] | ||
:'''Observations and Discussion''' | :'''Observations and Discussion''' | ||
:*[http://wiki.yeastgenome.org/index.php/CommunityW303.html CSY3] | :*[http://wiki.yeastgenome.org/index.php/CommunityW303.html CSY3] | ||
| + | |||
| + | ===Tissue Culture room=== | ||
| + | :*[[Smolke:Protocols/Adherent cell care and feeding|Adherent cell care and feeding]] | ||
| + | :*[[Smolke:Protocols/Media stocks|Media stocks]] | ||
| + | :*[[Smolke:Protocols/Freezer stock management and database|Freezer stock management and database]] | ||
| + | :*[[Smolke:Protocols/General Maintenance|General maintenance]] | ||
| + | :*[[Smolke:Protocols/General Sterility|General sterility]] | ||
| + | |||
| + | ===Quanta=== | ||
| + | :*[[Smolke:Protocols/Preventing Clogs|Preventing Clogs]] | ||
| + | :*[[Smolke:Protocols/Clearing Clogs|Clearing Clogs]] | ||
| + | |||
| + | ===VYB=== | ||
| + | :*[[Smolke:Protocols/Guidelines|Guidelines]] | ||
===Miscellaneous=== | ===Miscellaneous=== | ||
:*[[Smolke:Protocols/Glass Beads|Glass Beads]] | :*[[Smolke:Protocols/Glass Beads|Glass Beads]] | ||
:*[[Smolke:Protocols/Printing Posters|Poster Printing]] | :*[[Smolke:Protocols/Printing Posters|Poster Printing]] | ||
| + | :*[[Smolke:Protocols/Pipet-Aid|My pipet-aid is broken!]] | ||
| + | :*[[Smolke:Protocols/Cleaning Bottles for the LC|Cleaning Bottles for the LC]] | ||
==Solutions== | ==Solutions== | ||
| - | + | *[[Smolke:Protocols/Pfu Buffer|Pfu Buffer]] | |
| - | + | *[[Smolke:Protocols/Taq Buffer|Taq Buffer]] | |
| - | + | *[[Smolke:Protocols/DO Media|10x DO Media]] | |
| - | + | *[[Smolke:Protocols/dNTP Mixture|dNTP Mixture]] | |
| + | *[[Smolke:Protocols/5FOA|5-Fluoroorotic acid]] | ||
| + | *[[Smolke:Protocols/Antibiotics|Antibiotic Stocks]] | ||
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