Smolke:Protocols

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(Yeast)
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:*[[Smolke:Protocols/Gel extraction|Gel extraction]]
:*[[Smolke:Protocols/Gel extraction|Gel extraction]]
:*[[Smolke:Protocols/Ligation|Ligation]]
:*[[Smolke:Protocols/Ligation|Ligation]]
-
:*[[Smolke:Protocols/Transformation|Transformation]]
+
:*[[Smolke:Protocols/Transformation|Electrocompetent Transformation]]
 +
:*[[Smolke:Protocols/Heat Shock Transformation|Heat Shock Transformation]]
:*[[Smolke:Protocols/Screening|Colony PCR]]
:*[[Smolke:Protocols/Screening|Colony PCR]]
:*[[Smolke:Protocols/Digest Screening|Screening by digestion]]
:*[[Smolke:Protocols/Digest Screening|Screening by digestion]]
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:*[[Smolke:Protocols/Freezer stocks|Making freezer stocks]]
:*[[Smolke:Protocols/Freezer stocks|Making freezer stocks]]
:*[[Smolke:Protocols/Gene assembly|Gene assembly]]
:*[[Smolke:Protocols/Gene assembly|Gene assembly]]
 +
:*[[Smolke:Protocols/EtBr stock|EtBr stock]]
===Yeast===
===Yeast===
:'''Protocols'''
:'''Protocols'''
 +
:*[[Smolke:Protocols/Yeast Media Recipes | Yeast Media Recipes]]
:*[[Smolke:Protocols/Dropout plates|Pouring yeast dropout plates]]
:*[[Smolke:Protocols/Dropout plates|Pouring yeast dropout plates]]
:*[[Smolke:Protocols/Yeast transformation|Yeast plasmid transformation]]
:*[[Smolke:Protocols/Yeast transformation|Yeast plasmid transformation]]
 +
:*[[Smolke:Protocols/Quick yeast transformation|Quick Yeast plasmid transformation]]
:*[[Smolke:Protocols/Yeast Colony PCR|Yeast colony PCR]]
:*[[Smolke:Protocols/Yeast Colony PCR|Yeast colony PCR]]
:*[[Smolke:Protocols/Western|Anti-V5 Western]]
:*[[Smolke:Protocols/Western|Anti-V5 Western]]
-
:*[[Smolke:Protocols/Library_transformation|Library-scale yeast transformations]]
+
:*[[Smolke:Protocols/Library_transformation|Library-scale yeast transformations & Gap repair]]
:*[[Smolke:Protocols/Chromosomal Modification|Yeast chromosomal modifications]]
:*[[Smolke:Protocols/Chromosomal Modification|Yeast chromosomal modifications]]
:*[[Smolke:Protocols/Yeast plasmid prep|Yeast plasmid prep]]
:*[[Smolke:Protocols/Yeast plasmid prep|Yeast plasmid prep]]
 +
:*[[Smolke:Protocols/Feeding yeast cultures|Feeding yeast cultures]]
:*[[Smolke:Protocols/Yeast metabolite extraction|Yeast metabolite extraction]]
:*[[Smolke:Protocols/Yeast metabolite extraction|Yeast metabolite extraction]]
 +
:*[[Smolke:Protocols/Heme measurements|Heme measurements]]
:'''Observations and Discussion'''
:'''Observations and Discussion'''
:*[http://wiki.yeastgenome.org/index.php/CommunityW303.html CSY3]
:*[http://wiki.yeastgenome.org/index.php/CommunityW303.html CSY3]
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:*[[Smolke:Protocols/Preventing Clogs|Preventing Clogs]]
:*[[Smolke:Protocols/Preventing Clogs|Preventing Clogs]]
:*[[Smolke:Protocols/Clearing Clogs|Clearing Clogs]]
:*[[Smolke:Protocols/Clearing Clogs|Clearing Clogs]]
 +
 +
===VYB===
 +
:*[[Smolke:Protocols/Guidelines|Guidelines]]
===Miscellaneous===
===Miscellaneous===

Revision as of 15:30, 16 October 2012

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