Smolke:Protocols: Difference between revisions
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==Protocols== | ==Protocols== | ||
=== | ===Cloning=== | ||
:*[[Smolke:Protocols/Primer Design|Primer Design]] | |||
:*[[Smolke:Protocols/Plasmid prep|Plasmid prep with Epoch columns and homemade buffers]] | |||
:*[[Smolke:Protocols/Insert prep|PCR amplifying inserts from plasmids]] | |||
:*[[Smolke:Protocols/DNA purification|When to use phenol-chloroform vs. columns]] | |||
:*[[Smolke:Protocols/Digests|Restriction digests]] | |||
:*[[Smolke:Protocols/Gel extraction|Gel extraction]] | |||
:*[[Smolke:Protocols/Ligation|Ligation]] | |||
:*[[Smolke:Protocols/Transformation|Electrocompetent Transformation]] | |||
:*[[Smolke:Protocols/Heat Shock Transformation|Heat Shock Transformation]] | |||
:*[[Smolke:Protocols/Screening|Colony PCR]] | |||
:*[[Smolke:Protocols/Digest Screening|Screening by digestion]] | |||
:*[[Smolke:Protocols/Sequencing|Sequencing with Elim]] | |||
:*[[Smolke:Protocols/Freezer stocks|Making freezer stocks]] | |||
:*[[Smolke:Protocols/DNA assembly|DNA assembly]] | |||
:*[[Smolke:Protocols/EtBr stock|EtBr stock]] | |||
====Observations and Discussion | ===Yeast=== | ||
:'''Protocols''' | |||
:*[[Smolke:Protocols/Yeast Media Recipes | Yeast Media Recipes]] | |||
:*[[Smolke:Protocols/Dropout plates|Pouring yeast dropout plates]] | |||
:*[[Smolke:Protocols/Yeast transformation|Yeast plasmid transformation]] | |||
:*[[Smolke:Protocols/Quick yeast transformation|Quick Yeast plasmid transformation]] | |||
:*[[Smolke:Protocols/Yeast Colony PCR|Yeast colony PCR]] | |||
:*[[Smolke:Protocols/Western|Anti-V5 Western]] | |||
:*[[Smolke:Protocols/Library_transformation|Library-scale yeast transformations & Gap repair]] | |||
:*[[Smolke:Protocols/Chromosomal Modification|Yeast chromosomal modifications]] | |||
:*[[Smolke:Protocols/Yeast plasmid prep|Yeast plasmid prep]] | |||
:*[[Smolke:Protocols/Feeding yeast cultures|Feeding yeast cultures]] | |||
:*[[Smolke:Protocols/Yeast metabolite extraction|Yeast metabolite extraction]] | |||
:*[[Smolke:Protocols/Heme measurements|Heme measurements]] | |||
:'''Observations and Discussion''' | |||
:*[http://wiki.yeastgenome.org/index.php/CommunityW303.html CSY3] | |||
=== | ===Tissue Culture room=== | ||
*[[Smolke: | :*[[Smolke:Protocols/Adherent cell care and feeding|Adherent cell care and feeding]] | ||
:*[[Smolke:Protocols/Media stocks|Media stocks]] | |||
:*[[Smolke:Protocols/Freezer stock management and database|Freezer stock management and database]] | |||
:*[[Smolke:Protocols/General Maintenance|General maintenance]] | |||
:*[[Smolke:Protocols/General Sterility|General sterility]] | |||
=== | ===Quanta=== | ||
*[[Smolke: | :*[[Smolke:Protocols/Preventing Clogs|Preventing Clogs]] | ||
:*[[Smolke:Protocols/Clearing Clogs|Clearing Clogs]] | |||
=== | ===VYB=== | ||
:*[[Smolke:Protocols/Guidelines|Guidelines]] | |||
=== | ===Miscellaneous=== | ||
:*[[Smolke:Protocols/Glass Beads|Glass Beads]] | |||
:*[[Smolke:Protocols/Printing Posters|Poster Printing]] | |||
*[[ | :*[[Smolke:Protocols/Pipet-Aid|My pipet-aid is broken!]] | ||
:*[[Smolke:Protocols/Cleaning Bottles for the LC|Cleaning Bottles for the LC]] | |||
:*[[Smolke:Protocols/96-well plates|96-well plates]] | |||
== | ==Solutions== | ||
*[[Smolke: | *[[Smolke:Protocols/Pfu Buffer|Pfu Buffer]] | ||
*[[Smolke: | *[[Smolke:Protocols/Taq Buffer|Taq Buffer]] | ||
*[[Smolke:Protocols/DO Media|10x DO Media]] | |||
*[[Smolke:Protocols/dNTP Mixture|dNTP Mixture]] | |||
*[[Smolke:Protocols/5FOA|5-Fluoroorotic acid]] | |||
*[[Smolke:Protocols/Antibiotics|Antibiotic Stocks]] |
Latest revision as of 16:21, 17 February 2014
ProtocolsCloning
Yeast
Tissue Culture roomQuantaVYBMiscellaneousSolutions |