Smolke:Protocols

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Note: If possible, please follow the standard protocol format. Protocols Cloning Plasmid prep with Epoch columns and homemade buffers PCR amplifying inserts from plasmids When to use phenol-chloroform vs. columns Restriction digests Gel extraction Ligation Transformation Colony PCR Screening by digestion Sequencing with Elim Making freezer stocks Yeast Protocols Pouring yeast dropout plates Colony PCR (Travis' version) Anti-V5 Western Library-scale yeast transformations Observations and Discussion CSY3 Miscellaneous Protocols Dishwasher Glass Beads Solutions Recipes Pfu Buffer Taq Buffer 10x DO Media dNTP Mixture