Smolke:Protocols

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Note: If possible, please follow the standard protocol format. Protocols Cloning Primer Design Plasmid prep with Epoch columns and homemade buffers PCR amplifying inserts from plasmids When to use phenol-chloroform vs. columns Restriction digests Gel extraction Ligation Transformation Colony PCR Screening by digestion Sequencing with Elim Making freezer stocks Yeast Protocols Pouring yeast dropout plates Yeast plasmid transformation Yeast Colony PCR Anti-V5 Western Library-scale yeast transformations Yeast chromosomal modifications Observations and Discussion CSY3 Miscellaneous Protocols Glass Beads Solutions Recipes Pfu Buffer Taq Buffer 10x DO Media dNTP Mixture