Smolke:Protocols/DNA purification: Difference between revisions
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*Cleaning up reverse transcription products during SELEX. --YC | *Cleaning up reverse transcription products during SELEX. --YC | ||
*When I'm in an absolute time crunch, which is not often. --YC | *When I'm in an absolute time crunch, which is not often. --YC | ||
*Beware the size of you products and whether it's double-stranded or not. I don't have much luck purifying RT products if it's close to 100bp long. --JL | |||
==Situations to use phenol-chloroform extraction & ethanol precipitation== | ==Situations to use phenol-chloroform extraction & ethanol precipitation== | ||
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*You could also just do ethanol precipitation without phenol-chloroform extraction if you are simply trying to reduce the volume of a sample (for subsequent gel extraction, for example). I believe some people also just put it in the speed vac for this purpose. --YC | *You could also just do ethanol precipitation without phenol-chloroform extraction if you are simply trying to reduce the volume of a sample (for subsequent gel extraction, for example). I believe some people also just put it in the speed vac for this purpose. --YC | ||
*Large quantities of DNA (>10ug). -JKM | *Large quantities of DNA (>10ug). -JKM | ||
Note: phenol-chloroform is stored at 4 °C |
Latest revision as of 21:24, 26 October 2011
Situations to use a column-based purification kit
Situations to use phenol-chloroform extraction & ethanol precipitation
Note: phenol-chloroform is stored at 4 °C |