Smolke:Protocols/DNA purification: Difference between revisions
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(New page: *Situations in which I (YC) would use a column-based purification kit **Cleaning up PCR products after each step in assembly PCR **Cleaning up reverse transcription products during SELEX *...) |
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Revision as of 10:45, 15 October 2009
- Situations in which I (YC) would use a column-based purification kit
- Cleaning up PCR products after each step in assembly PCR
- Cleaning up reverse transcription products during SELEX
- When I'm in an absolute time crunch, which is not often
- Situations in which I (YC) would use phenol-chloroform extraction & ethanol precipitation
- Cleaning up and concentrating PCR-amplified inserts for cloning
- Cleaning up after digestion
- You could also just do ethanol precipitation without phenol-chloroform extraction if you are simply trying to reduce the volume of a sample (for subsequent gel extraction, for example). I believe some people also just put it in the speed vac for this purpose.
