Smolke:Protocols/DO Media
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Recipe
Amino Acid
Stock Concentration
(g/L)
Volume Added
(mL/L)
Final Concentration
(mg/L)
Notes
Adenine sulfate
2
100
20
Stored at RT
Uracil
2
100
20
L-Tryptophan
10
20
20
L-Histidine hydrochloride
10
20
20
L-Arginine hydrochloride
10
20
20
L-Methionine
10
20
20
L-Tyrosine
2
150
30
add ~5mL 5N NaOH
L-Leucine
10
60
60
L-Isoleucine
10
30
30
L-Lysine hydrochloride
10
30
30
L-Phenylalanine
10
50
50
Stored at RT
L-Glutamic acid
10
100
100
Stored at RT
L-Aspartic acid
10
100
100
Stored at RT
L-Valine
30
50
150
L-Threonine
40
50
200
L-Serine
80
50
400
Nanopure Water
Remainder
Notes
Leave out whichever amino acid(s) you're dropping out, and replace the volume with water.
Stocks which are stored at RT are in the cabinet with autoclaved media.
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