Smolke:Protocols/DO Media
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Recipe
Amino Acid | Stock Concentration (mg/L) |
Volume Added (mL/L) |
Final Concentration (mg/L) |
Notes |
---|---|---|---|---|
Adenine sulfate | 200 | 100 | 20 | Stored at RT |
Uracil | 200 | 100 | 20 | |
L-Tryptophan | 1000 | 20 | 20 | |
L-Histidine hydrochloride | 1000 | 20 | 20 | |
L-Arginine hydrochloride | 1000 | 20 | 20 | |
L-Methionine | 1000 | 20 | 20 | |
L-Tyrosine | 200 | 150 | 30 | |
L-Leucine | 1000 | 60 | 60 | |
L-Isoleucine | 1000 | 30 | 30 | |
L-Lysine hydrochloride | 1000 | 30 | 30 | |
L-Phenylalanine | 1000 | 50 | 50 | Stored at RT |
L-Glutamic acid | 1000 | 100 | 100 | Stored at RT |
L-Aspartic acid | 1000 | 100 | 100 | Stored at RT |
L-Valine | 3000 | 50 | 150 | |
L-Threonine | 4000 | 50 | 200 | |
L-Serine | 8000 | 50 | 400 | |
Nanopure Water | Remainder |
Notes
- Leave out whichever amino acid(s) you're dropping out, and replace the volume with water.
- Stocks which are stored at RT are in the cabinet with autoclaved media.