Smolke:Protocols/Dropout plates: Difference between revisions
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==Notes== | ==Notes== | ||
*Check which kind of YNB you are using. If using the kind that just says w/o Amino acids (rather than w/o Amino Acids and Ammonium Sulfate), use 3.35g of that (rather than 0.85g), and don't add Ammonium Sulfate -Leo | *Check which kind of YNB you are using. If using the kind that just says w/o Amino acids (rather than w/o Amino Acids and Ammonium Sulfate--which I believe means, and should say, w/o Amino Acids *or* Ammonium Sulfate), use 3.35g/500mL final of that (rather than 0.85g), and don't add Ammonium Sulfate -Leo | ||
*Before autoclaving, the mixture will separate into two layers. It should go into solution after autoclaving. | *Before autoclaving, the mixture will separate into two layers. It should go into solution after autoclaving. | ||
*If pouring multiple bottles of media, the remaining bottles can stay in the autoclave until needed. | *If pouring multiple bottles of media, the remaining bottles can stay in the autoclave until needed. |
Latest revision as of 10:18, 28 August 2012
OverviewMaking selective yeast plates. Materials
ProcedureN.B. - this protocol makes ~1 sleeve of moderately thick plates. Adjust volumes as needed.
Notes
ContactFor another protocol, look here. |