Revision as of 22:53, 13 July 2012 by
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Making Ethidium Bromide stock
Concentrated stock (20,000x)
Make the stock in a 15 mL Falcon tube with three tablets of EtBr (50 mg for each, 150 mg in total) and 15 mL of H2O.
The final concentration for the concentrated EtBr stock is 1 mg/mL.
After you make the stock, wrap the tube up with tin foil and place it in the 4C deli fridge.
NOTE: Any tips or tubes that touch the concentrated stock are considered as hazardous wastes and should go into hazardous waste bottle by the gel station.
Working stock (5,000x)
Take 250 uL of the concentrated EtBr stock (20,000x) out and add 750 uL of H2O (a total volume of 1000 uL).
The working stock is not considered as hazardous waste. (Lower than 0.4%)
20 uL of working stock (5,000x) per 100 mL agarose gel.
Created by Yen-Hsiang (07/13/2012)
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