Smolke:Protocols/Feeding yeast cultures: Difference between revisions
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m (New page: {{Smolke_Top}} ==Overview== This protocol is used to set up cultures, feed substrates, and harvest metabolites from media for analysis by LCMS. ==Procedure== ===Materials=== * substrate ...) |
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* selective NINR media with dextrose replacing the normal sugars and without water added | * selective NINR media with dextrose replacing the normal sugars and without water added | ||
* selective yeast media | * selective yeast media | ||
* syringe filter & syringe | |||
* culture plates | * culture plates | ||
* Agilent LC plates | |||
===Method=== | ===Method=== | ||
====Making Substrate Stocks=== | ====Making Substrate Stocks==== | ||
*Norlaudanosoline: 20mM stock (5X) (4mM working concentration) | *Norlaudanosoline: 20mM stock (5X) (4mM working concentration) | ||
**Location: Chemical cabinet, labeled (+/-) tetrahydropapaveroline hydrobromide | **Location: Chemical cabinet, labeled (+/-) tetrahydropapaveroline hydrobromide | ||
Line 29: | Line 31: | ||
**Storage: 4C | **Storage: 4C | ||
====Growing and Feeding Cultures=== | ====Growing and Feeding Cultures==== | ||
Day 1: | Day 1: | ||
*Set up an overnight culture of strains in selective media (3mL). (Use synthetic complete in place of YPD) | *Set up an overnight culture of strains in selective media (3mL). (Use synthetic complete in place of YPD) |
Latest revision as of 20:15, 11 January 2012
OverviewThis protocol is used to set up cultures, feed substrates, and harvest metabolites from media for analysis by LCMS. ProcedureMaterials
MethodMaking Substrate Stocks
Growing and Feeding CulturesDay 1:
Day 2:
Analysis
ReferencesContact |