Smolke:Protocols/Gel extraction: Difference between revisions
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(New page: *Comb size **Single wells from the smallest comb (15 wells per row) can hold up to 20 ul **Single wells from un-taped medium comb (8 wells per row) can hold up to 50 ul *Agarose density **...) |
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Revision as of 11:32, 15 October 2009
- Comb size
- Single wells from the smallest comb (15 wells per row) can hold up to 20 ul
- Single wells from un-taped medium comb (8 wells per row) can hold up to 50 ul
- Agarose density
- Above 3 kb: 0.8%
- 1-3 kb: 1%
- 250-1000 bp: 1.5%
- Below 250 bp: 2%
- 75V for 45 min, or 85V for 35 min
- Minimizing the amount of agarose in the cutout band seems to improve yields
- I (YC) clean the razor blade between each use with a Kimwipe and do not change blades until it is dull or known to be contaminated. I have never had cross-contamination in my gel extraction doing it this way.
