Smolke:Protocols/Gel extraction

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Comb size Single wells from the smallest comb (15 wells per row) can hold up to 20 ul Single wells from un-taped medium comb (8 wells per row) can hold up to 50 ul Agarose density Above 3 kb: 0.8% 1-3 kb: 1% 250-1000 bp: 1.5% Below 250 bp: 2% 75V for 45 min, or 85V for 35 min Minimizing the amount of agarose in the cutout band seems to improve yields Notes I clean the razor blade between each use with a Kimwipe and do not change blades until it is dull or known to be contaminated. I have never had cross-contamination in my gel extraction doing it this way. --YC