Smolke:Protocols/Heat Shock Transformation: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
(One intermediate revision by one other user not shown) | |||
Line 4: | Line 4: | ||
**1 tube of thawed chemically competent cells (keep covered in ice at all times) | **1 tube of thawed chemically competent cells (keep covered in ice at all times) | ||
**1-10 uL ligation product | **1-10 uL ligation product | ||
** | **250 uL SOC | ||
**1 LB plate with appropriate antibiotics | **1 LB plate with appropriate antibiotics | ||
*Procedure | *Procedure | ||
**Add DNA to cells | **Add DNA to cells | ||
**Incubate on ice for 0-20 minutes | **Incubate on ice for 0-20 minutes | ||
**Heat shock at | **Heat shock at 42 °C for 30 seconds | ||
**Add SOC | **Incubate on ice for 0-2 minutes | ||
**Incubate on rotator in | **Add SOC | ||
** | **Incubate on rotator in 37 °C incubator for 0-60 minutes | ||
**Plate 50-250 uL |
Latest revision as of 18:07, 14 February 2013
|