Smolke:Protocols/Heat Shock Transformation: Difference between revisions
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**Incubate on ice for 0-20 minutes | **Incubate on ice for 0-20 minutes | ||
**Heat shock at 42 °C for 30 seconds | **Heat shock at 42 °C for 30 seconds | ||
**Incubate on ice for 2 minutes | **Incubate on ice for 0-2 minutes | ||
**Add SOC | **Add SOC | ||
**Incubate on rotator in 37 °C incubator for 0-60 minutes | **Incubate on rotator in 37 °C incubator for 0-60 minutes | ||
**Plate 50-250 uL | **Plate 50-250 uL |
Latest revision as of 18:07, 14 February 2013
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