Smolke:Protocols/Insert prep: Difference between revisions
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==Protocol== | ==Protocol== | ||
*Prepare 5 x 100-ul PCR reactions for each insert | *Prepare 5 x 100-ul PCR reactions for each insert (see notes below on reaction volume) | ||
**400 ul water | **400 ul water | ||
**50 ul 10x Taq buffer | **50 ul 10x Taq buffer |
Revision as of 14:31, 15 October 2009
Protocol
Notes
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