Smolke:Protocols/Ligation: Difference between revisions
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**My point is that, in my experience, the cloning will either work or not work, and concentrating the ligation has not made a deference. --YC | **My point is that, in my experience, the cloning will either work or not work, and concentrating the ligation has not made a deference. --YC | ||
*If you do wish to concentrate the ligation, make sure you either do phenol-chloroform extraction or use a column and not just concentrate with the speed vac. | *If you do wish to concentrate the ligation, make sure you either do phenol-chloroform extraction or use a column and not just concentrate with the speed vac. | ||
*If you're not cleaning up the ligation, it should be heat inactivated (65C for 10-20 minutes) before transformation. -Josh |
Revision as of 14:28, 15 October 2009
General setup
Reaction time
Concentration and purification after ligation
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