Smolke:Protocols/Media stocks

From OpenWetWare

< Smolke:Protocols
Revision as of 20:39, 11 July 2012 by James V. Vowles (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

Home        Contact        Internal        Protocols        Lab Members        Publications        Research       


  • When aliquoting media stock make sure to leave some empty volume in the tube (i.e., fill a 50-ml Falcon to only the 40-ml mark)
    • This is especially important for trypsin and FBS aliquots, which will expand when frozen.
  • Each researcher should have their own aliquoted stock of media.
  • Take responsibility for aliquoting new stocks, when older stocks are almost used up. Make sure to use sterile technique in preparing aliquots.
Personal tools